期刊
TOXICOLOGY IN VITRO
卷 22, 期 3, 页码 716-722出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2007.11.012
关键词
ecotoxicology; phytoplankton; physiology; irrigated rice; basagran
类别
Chlorophyll fluorescence techniques are used for the detection of toxic substances in samples of photosynthetic cells by measuring chlorophyll a fluorimetric parameters, which are a response of the PSII physiological status. This work was conducted to determine the effects of the herbicide bentazon (CASRN 25057-89-0) on growth and maximum quantum yield of photosystem II (Fv/Fm) in cells of the marine diatom Skeletonema costatum. Unialgal cultures were exposed to several bentazon concentrations and its effects on algal growth and Fv/Fm were determined. The traditional algal growth inhibition test (algal biomass measurements) and DCMU-induced chlorophyll a variable fluorescence measurements were determined. Our results showed that even low concentrations of bentazon rapidly lead to Fv/Fm decrease, while the effects on algal growth were detected after 24 h of exposure. The LOEC (2.81 mg L-1) and EC50 (13.0 mg L-1) determined through Fv/Fm experiments were lower than the LOEC (22.5 mg L-1) and EC50 (24.0 mg L-1) determined through algal growth inhibition experiments. This confirms that the Fv/Fm is a more sensitive parameter than algal growth for monitoring the effects of bentazon. The present results have demonstrated the applicability of Fv/Fm parameter to access the early toxicity of bentazon, as well as other PSII-inhibition compounds, before significant changes occurred in the original concentration and bioavailability of these toxicants during longer exposure times. (C) 2007 Elsevier Ltd. All rights reserved.
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