期刊
TOXICOLOGY IN VITRO
卷 22, 期 5, 页码 1387-1391出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2008.02.022
关键词
chlorpyrifos; chlorpyrifos oxon; C6 cells; differentiation; microtubule proteins
类别
The aim of this work was to assess the toxic effects of the phosphorothionate insecticide chlorpyrifos (CPF) and its major in vivo metabolite chlorpyrifos oxon (CPO) on differentiating rat C6 glioma cells. At sublethal concentrations (1 -10 mu M), both compounds were able to inhibit the development of extensions from C6 cells induced to differentiate by sodium butyrate.. Western blot analysis of C6 cell lysates revealed that 4 h exposure to CPF was associated with decreased levels of the cytoskeletal protein MAPI B compared to controls, whereas the levels of the cytoskeletal proteins tubulin and MAP2c were not significantly affected. Western blot analysis of extracts of cells treated with CPO showed a significant, concentration-dependent decrease in the levels of tubulin after 24 h. MAP-1B levels were also significantly decreased. The above changes were not temporally related to acetylcholinesterase (AChE) inhibition. These results suggest that both CPF and CPO can exert toxic effects directly on glial cell differentiation and that the latter compound has a potent effect on the microtubule network. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据