期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 248, 期 1, 页码 28-37出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2010.07.008
关键词
Co-exposure; Di(n-butyl)phthalate; Benzo(a)pyrene; Testicular macrophages; IL-1 beta; Testosterone
资金
- National Natural Science Foundation of China [30630056]
- Ministry of Science and Technology of China [2003BA869C]
Di(n-butyl)phthalate (DBP) and benzo(a)pyrene (BaP) are environmental endocrine disruptors that are potentially hazardous to humans. These chemicals affect testicular macrophage immuno-endocrine function and testosterone production. However, the underlying mechanisms for these effects are not fully understood. It is well known that interleukin-1 beta (IL-1 beta), which is secreted by testicular macrophages, plays a trigger role in regulating Leydig cell steroidogenesis. The purpose of this study was to reveal the effects of co-exposure to DBP and BaP on testicular macrophage subset expression, IL-1 beta secretion and testosterone production. Adult male Sprague-Dawley rats were randomly divided into seven groups; two groups received DBP plus BaP (DBP + BaP: 50 + 1 or 250 + 5 mg/kg/day) four groups received DBP or BaP alone (DBP: 50 or 250 mg/kg/day; BaP: 1 or 5 mg/kg/day), and one group received vehicle alone (control). After co-exposure for 90 days, the relative expression of macrophage subsets and their functions changed. ED2+ testicular macrophages (reactive with a differentiation-related antigen present on the resident macrophages) were activated and IL-1 beta secretion was enhanced. DBP and BaP acted additively, as demonstrated by greater IL-1 beta secretion relative to each compound alone. These observations suggest that exposure to DBP plus BaP exerted greater suppression on testosterone production compared with each compound alone. The altered balance in the subsets of testicular macrophages and the enhanced ability of resident testicular macrophages to secrete IL-1 beta, resulted in enhanced production of IL-1 beta as a potent steroidogenesis repressor. This may represent an important mechanism by which DBP and BaP repress steroidogenesis. (C) 2010 Elsevier Inc. All rights reserved.
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