期刊
PLOS GENETICS
卷 11, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1004905
关键词
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资金
- National Institutes of Health [R01GM070795, R01GM059138]
- Spanish Ministry of Economy and Competitiveness/European Regional Development Fund [BFU2013-43269-P]
- Junta de Andalucia [P11-CVI-7576]
- Chinese Academy of Sciences
- Peking-Tsinghua Center for Life Science
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM059138, R01GM070795] Funding Source: NIH RePORTER
Active DNA demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases. Active DNA demethylation in animals requires the DNA glycosylase TDG or MBD4, which functions after oxidation or deamination of 5-methylcytosine, respectively. However, little is known about the steps following DNA glycosylase action in the active DNA demethylation pathways in plants and animals. We show here that the Arabidopsis APE1L protein has apurinic/apyrimidinic endonuclease activities and functions downstream of ROS1 and DME. APE1L and ROS1 interact in vitro and co-localize in vivo. Whole genome bisulfite sequencing of ape1l mutant plants revealed widespread alterations in DNA methylation. We show that the ape1l/zdp double mutant displays embryonic lethality. Notably, the ape1l(+/-) zdp(-/-) mutant shows a maternal-effect lethality phenotype. APE1L and the DNA phosphatase ZDP are required for FWA and MEA gene imprinting in the endosperm and are important for seed development. Thus, APE1L is a new component of the active DNA demethylation pathway and, together with ZDP, regulates gene imprinting in Arabidopsis.
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