期刊
TOXICOLOGY
卷 291, 期 1-3, 页码 83-92出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2011.11.001
关键词
Bisphenol A; Zebrafish; Behavior; Learning; Hyperactivity; Endocrine disruptor
资金
- National Institutes of Health [T32ES7060, P30 ES000210, R21ES018970]
- NIEHS Children's Environmental Health Sciences Core
- United States Environmental Protection Agency (EPA)
Developmental bisphenol A (BPA) exposure has been implicated in adverse behavior and learning deficits. The mode of action underlying these effects is unclear. The objectives of this study were to identify whether low-dose, developmental BPA exposure affects larval zebrafish locomotor behavior and whether learning deficits occur in adults exposed during development. Two control compounds, 17 beta-estradiol (an estrogen receptor ligand) and GSK4716 (a synthetic estrogen-related receptor gamma ligand), were included. Larval toxicity assays were used to determine appropriate BPA, 17 beta-estradiol, and GSK4716 concentrations for behavior testing. BPA tissue uptake was analyzed using HPLC and lower doses were extrapolated using a linear regression analysis. Larval behavior tests were conducted using a ViewPoint Zebrabox. Adult learning tests were conducted using a custom-built T-maze. BPA exposure to <30 mu M was non-teratogenic. Neurodevelopmental BPA exposure to 0.01, 0.1, or 1 mu M led to larval hyperactivity or learning deficits in adult zebrafish. Exposure to 0.1 mu M 17 beta-estradiol or GSK4716 also led to larval hyperactivity. This study demonstrates the efficacy of using the zebrafish model for studying the neurobehavioral effects of low-dose developmental BPA exposure. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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