期刊
TOXICOLOGY
卷 288, 期 1-3, 页码 27-33出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2011.06.014
关键词
Antimicrobial peptide; Polybia-CP; Molecular dynamics simulations; Membrane perturbation; Carpet model
资金
- National Natural Science Foundation of China [90813012, 20932003]
- Ministry of Science and Technology [2009ZX09503-017]
- Ministry of Education of China [2010082]
- Fundamental Research Funds for the Central Universities 369 [lzujbky-2010-129]
Antimicrobial peptide polybia-CP was purified from the venom of the social wasp Polybia paulista. it has an amphipathic sequence ILGTILGLLKSL-NH2 and possesses potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this study we synthesized polybia-CP, studied its cytotoxity on tumor cells and proposed its possible mechanism. Our results revealed that polybia-CP exerts its cytotoxic efficacy by disrupting the integrity of cell membrane. Furthermore, molecular dynamics (MD) simulations were employed to investigate the mechanism of membrane perturbation. Both the MD simulations and the experimental data indicated that polybia-CP takes a standard a-helix conformation in the membrane. These findings together with the other experimental results support a speculation of mechanism similar to the carpet model. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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