期刊
TOXICOLOGY
卷 272, 期 1-3, 页码 52-58出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2010.04.005
关键词
BPA; Development; MAP2; Synapse; Synapsin I; Hypothalamus; ERK
资金
- Ministry of Education, Cultures, Sports, Science and Technology of Japan [17500241]
- Grants-in-Aid for Scientific Research [17500241] Funding Source: KAKEN
Estradiol plays an essential role in sexual differentiation of the rodent hypothalamus. Endocrine disruptors with estrogenic activity such as bisphenol A (BPA) are reported to disturb sexual differentiation of the hypothalamus The purpose of the present study was to examine in vitro effects of BPA on developing hypothalamic neurons by focusing on a presynaptic protein synapsin I and microtubule-associated protein 2 (MAP2). In cultured hypothalamic cells from fetal rats, treatment with BPA enhanced both dendritic and synaptic development, as evidenced by increases in the area of dot-like staining of synapsin I and MAP2-positive area An estrogen receptor (ER) antagonist, ICI 182,780, only partially blocked BPA-induced increase in the synapsin I-area, while it suppressed the MAP2-area increased by BPA A specific ERK inhibitor, U0126, reduced the synapsin I-area without affecting the MAP2-area BPA significantly decreased protein levels of synapsin I phosphorylated at Ser-9 and Ser-603 These findings indicate that BPA-inducing effects on dendritic and synaptic development arc mediated by different molecular pathways (C) 2010 Elsevier Ireland Ltd. All rights reserved
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