期刊
PLOS GENETICS
卷 11, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1004975
关键词
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资金
- AGENCIA NACIONAL DE PROMOCION CIENTIFICA Y TECNICA(ANPCyT) [PICT2011-1610]
- CONICET, CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
- Deutscher Akademischer Austauschdienst (DAAD)
DNA replication is a key process in living organisms. DNA polymerase alpha (Pol alpha) initiates strand synthesis, which is performed by Pol epsilon and Pol delta in leading and lagging strands, respectively. Whereas loss of DNA polymerase activity is incompatible with life, viable mutants of Pol alpha and Pol epsilon were isolated, allowing the identification of their functions beyond DNA replication. In contrast, no viable mutants in the Pol delta polymerase-domain were reported in multicellular organisms. Here we identify such a mutant which is also thermosensitive. Mutant plants were unable to complete development at 28 degrees C, looked normal at 18 degrees C, but displayed increased expression of DNA replication-stress marker genes, homologous recombination and lysine 4 histone 3 trimethylation at the SEPALLATA3 (SEP3) locus at 24 degrees C, which correlated with ectopic expression of SEP3. Surprisingly, high expression of SEP3 in vascular tissue promoted FLOWERING LOCUS T (FT) expression, forming a positive feedback loop with SEP3 and leading to early flowering and curly leaves phenotypes. These results strongly suggest that the DNA polymerase delta is required for the proper establishment of transcriptionally active epigenetic marks and that its failure might affect development by affecting the epigenetic control of master genes.
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