期刊
TOXICOLOGICAL SCIENCES
卷 141, 期 1, 页码 278-291出版社
OXFORD UNIV PRESS
DOI: 10.1093/toxsci/kfu125
关键词
Drosophila; male infertility; endocrine disruption; phthalate; estrogen related receptor
类别
资金
- Council for Scientific and Industrial Research (CSIR), New Delhi [BSC0103]
- University Grants Commission (UGC), New Delhi [GAP 155]
- Science and Engineering Research Board (SERB), New Delhi [SR/FT/LS-133/2009]
Men are at risk of becoming completely infertile due to innumerable environmental chemicals and pollutants. These xenobiotics, hence, should be tested for their potential adverse effects on male fertility. However, the testing load, a monumental challenge for employing conventional animal models, compels the pursuit of alternative models. Towards this direction, we show here that Drosophila melanogaster, an invertebrate, with its well characterized/conserved male reproductive processes/proteome, recapitulates male reproductive toxicity phenotypes observed in mammals when exposed to a known reproductive toxicant, dibutyl phthalate (DBP). Analogous to mammals, exposure to DBP reduced fertility, sperm counts, seminal proteins, increased oxidative modification/damage in reproductive tract proteins and altered the activity of a hormone receptor (estrogen related receptor) in Drosophila males. In addition, we show here that DBP is metabolized to monobutyl phthalate (MBP) in exposed Drosophila males and that MBP is more toxic than DBP, as observed in higher organisms. These findings suggest Drosophila as a potential alternative to traditional animal models for the prescreening of chemicals for their reproductive adversities and also to gain mechanistic insights into chemical-mediated endocrine disruption and male infertility.
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