期刊
TOXICOLOGICAL SCIENCES
卷 135, 期 1, 页码 63-71出版社
OXFORD UNIV PRESS
DOI: 10.1093/toxsci/kft131
关键词
miR-1a; miRNA; pulmonary carcinogenesis; urethane
类别
资金
- National Key Basic Research Program of China [2012CB525004]
- National Natural Science Foundation of China [30972443]
- Research Fund for Doctoral Program of Higher Education of China [20114423110002]
- Key Program of Guangdong Natural Science Foundation [9251018201000004]
- University Talent Program of Guangdong [2010-79]
- University Talent Program of Guangzhou [10A003D]
- Science and Technology Project of Guangzhou [2010Y1-C441]
MicroRNAs (miRNAs) are small RNA molecules that regulate posttranscriptional gene expression. Previous research has suggested that aberrant miRNA expression often plays a critical role in many types of cancer, including lung cancer. However, the exact miRNAs that are involved in pulmonary carcinogenesis remain unclear. We investigated the miRNA-based molecular changes that occur in urethane-induced carcinogenicity and identified specific miRNA deregulation in pulmonary carcinogenesis induced by urethane. In this study, we used a lung cancer model in which Balb/c mice were exposed to urethane via ip injection once a week for four consecutive weeks. The mice were then killed in weeks 6, 12, or 24. Two small RNA libraries were constructed with the total RNA from the lung tumor and normal adjacent lung tissues of the urethane-injected mice collected in week 24. Using Solexa sequencing, we identified a plethora of differentially expressed miRNAs and predicted nine novel miRNAs. Further analysis demonstrated the sustainable downregulation of miR-1a in the lung tissues in lung carcinogenesis induced by urethane. The levels of miR-1a were also reduced in the serum. Our findings indicate that urethane exposure alters the expression of a cluster of miRNAs. The simultaneous downregulation of miR-1a in lung tissues and serum in urethane-induced pulmonary carcinogenesis suggests that miR-1a is associated with tumorigenesis.
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