期刊
TOPICS IN CATALYSIS
卷 55, 期 16-18, 页码 1146-1156出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11244-012-9893-0
关键词
Specific enzyme immobilization; Nanomaterials; Affinity tag; Click chemistry; Polyhistidine; Biotin; Non-covalent
Nanotechnology-inspired biocatalysts have aroused much interest recently because nanomaterials can provide the upper limits on enzyme-efficiency-determining factors. Non-specific immobilization approaches often face problems of enzyme leaching, 3D structure change and mass transfer resistance. Here, we focus on the review of specific enzyme immobilization approaches and have separated them into non-covalent and covalent categories.
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