期刊
TISSUE ENGINEERING PART A
卷 20, 期 11-12, 页码 1612-1620出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tea.2013.0307
关键词
-
资金
- Medical Engineering Solutions in the Osteoarthritis Centre of Excellence - Wellcome Trust
- EPSRC [088844/Z/09/Z]
- Royal Society Wolfson Research Merit Award
Tissue-engineering strategies for the treatment of osteoarthritis would benefit from the ability to induce chondrogenesis in precursor cells. One such cell source is bone marrow-derived stromal cells (BMSCs). Here, we examined the effects of moderate-strength static magnetic fields (SMFs) on chondrogenic differentiation in human BMSCs in vitro. Cells were cultured in pellet form and exposed to several strengths of SMFs for various durations. mRNA transcript levels of the early chondrogenic transcription factor SOX9 and the late marker genes ACAN and COL2A1 were determined by reverse transcription-polymerase chain reaction, and production of the cartilage-specific macromolecules sGAG, collage type 2 (Col2), and proteoglycans was determined both biochemically and histologically. The role of the transforming growth factor (TGF)-beta signaling pathway was also examined. Results showed that a 0.4 T magnetic field applied for 14 days elicited a strong chondrogenic differentiation response in cultured BMSCs, so long as TGF-beta 3 was also present, that is, a synergistic response of a SMF and TGF-beta 3 on BMSC chondrogenic differentiation was observed. Further, SMF alone caused TGF-beta secretion in culture, and the effects of SMF could be abrogated by the TGF-beta receptor blocker SB-431542. These data show that moderate-strength magnetic fields can induce chondrogenesis in BMSCs through a TGF-beta-dependent pathway. This finding has potentially important applications in cartilage tissue-engineering strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据