期刊
TISSUE ENGINEERING PART A
卷 15, 期 2, 页码 367-375出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tea.2008.0133
关键词
-
资金
- Royal Netherlands Academy of Arts and Sciences [06CDP030]
The objective of this study was to examine hard tissue formation of STRO-1-selected rat dental pulp-derived stem cells, seeded into a calcium phosphate ceramic scaffold, and implanted subcutaneously in mice. Previously, STRO-1 selection was used to obtain a mesenchymal stem cell progenitor subpopulation from primary dental pulp-derived stem cells. In the current study, these cells were cultured with three different media: BMP-plus'' medium containing dexamethasone and 100 ng/mL of rhBMP-2, odontogenic'' medium containing dexamethasone, and control'' medium without supplements. The cell-scaffold complexes were cultured in these media for 1, 4, or 8 days before implantation. Histological analysis demonstrated that the cultures with BMP-plus and 4 days of culture gave the highest percentage of hard tissue formation per implant (36 +/- 9% of pore area). Real-time PCR confirmed these results. In conclusion, STRO-1-selected dental pulp stem cells show effective hard tissue formation in vivo, and a short in vitro culture period and addition of BMP-2 can enhance this effect.
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