Development and Characterization of Heparin Immobilized Bacterial Cellulose(BC) for bone Tissue Engineering using Gamma-irradiation

Bacterial cellulose(BC) scaffolds are generated from gel by gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure and the ultrafme highly nanofibrous structure of mimic natural extra celluar matrix(ECM) for tissue engineering. In this study, 2-aminoethyl methacrylate(AEMA) was grafted onto BC surfade using gamma-irradiation and then heparin immobilized it using EDC/NHS reaction. The surface morphology of scaffolds were confirmed BC membranes by SEM for heparin and AEMA grafted it, so that it was not changed surface morphology. The result of TBO, fluorescamine staining, ATR-FTIR confirmed that heparin and AEMA immobilized it. Also result of in vitro test, heparin immobilized AEMA-BC proliferation of hMSC to higher than unmodified BC by CCK-8 and ALP activity then heparin immobilized AEMA-BC cumulative of BM-P-2 was confirmed decrease by release test. Therefore, heparin and AEMA grafted BC scaffolds have been possible to tissue scaffold in bone tissue engineering.