期刊
TISSUE ANTIGENS
卷 81, 期 6, 页码 428-434出版社
WILEY-BLACKWELL
DOI: 10.1111/tan.12113
关键词
cancer stem cell; cancer/testis gene; cancer/testis/stem gene
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [16209013, 17016061, 15659097]
- Japan Science and Technology Agency
- Ministry of Health, Labor and Welfare of Japan [15-17, 19-14]
- Ono Cancer Research Fund
- Takeda Science Foundation
- National Cancer Center Research and Development Fund [23-A-44]
- Grants-in-Aid for Scientific Research [16209013, 24791467, 24590449, 15659097, 17016061, 24659165, 23590427] Funding Source: KAKEN
Cancer/testis (CT) antigens encoded by CT genes are immunogenic antigens, and the expression of CT gene is strictly restricted to only the testis among mature organs. Therefore, CT antigens are promising candidates for cancer immunotherapy. In a previous study, we identified a novel CT antigen, DNAJB8. DNAJB8 was found to be preferentially expressed in cancer stem-like cells (CSCs)/cancer-initiating cells (CICs), and it is thus a novel CSC antigen. In this study, we hypothesized that CT genes are preferentially expressed in CSCs/CICs rather than in non-CSCs/-CICs and we examined the expression of CT genes in CSCs/CICs. The expression of 74 CT genes was evaluated in side population (SP) cells (=CSC) and main population (MP) cells (=non-CSC) derived from LHK2 lung adenocarcinoma cells, SW480 colon adenocarcinoma cells and MCF7 breast adenocarcinoma cells by RT-PCR and real-time PCR. Eighteen genes (MAGEA2, MAGEA3, MAGEA4, MAGEA6, MAGEA12, MAGEB2, GAGE1, GAGE8, SPANXA1, SPANXB1, SPANXC, XAGE2, SPA17, BORIS, PLU-1, SGY-1, TEX15 and CT45A1) showed higher expression levels in SP cells than in MP cells, whereas 10 genes (BAGE1, BAGE2, BAGE4, BAGE5, XAGE1, LIP1, D40, HCA661, TDRD1 and TPTE) showed similar expression levels in SP cells and MP cells. Thus, considerable numbers of CT genes showed preferential expression in CSCs/CICs. We therefore propose a novel sub-category of CT genes in this report: cancer/testis/stem (CTS) genes.
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