Effect of deltamethrin on the gills of Aphanius dispar: A microscopic study

Aphanius dispar, a freshwater fish (mean total length +/- sd, 3.42 +/- 0.33 cm) was exposed to different concentrations of deltamethrin (2.25, 2.50 and 3.00 mu g L-1), an insecticide used to control Dubas bug, Ommatissus lybicus in Oman. The histopathological changes in the structure of the gills in fish exposed to deltamethrin was studied using light, transmission and scanning electron microscopy. In light microscopy, the fish exposed to 2.25 mu g L-1 deltamethrin, showed hypertrophy and hyperplasia of chloride cells, while in 2.50 mu g L-1, additional changes like vacuolization, lifting of the lamellar epithelium and fusion of secondary lamellae were observed. The most severe alteration including vacuolization, desquamation of cells and dilation of intercellular space was recorded at 3.00 mu g L-1. Transmission and scanning electron microscopy, supports the findings of light microscopy and further document the ultrastructural damage caused, especially in exposure to 2.50 and 3.00 mu g L-1. Al l changes observed are described in detail. The deltamethrin exposure concentration of 2.25 mu g L-1 seems to be a threshold above which any small increase in concentration results in severe damage. All present results have been compared with those of previous studies on gill damage caused by various chemical substances. The impacts of the damage on the functioning of gills as a respiratory organ are discussed. (C) 2011 Elsevier Ltd. All rights reserved.