期刊
THROMBOSIS RESEARCH
卷 127, 期 6, 页码 551-559出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.thromres.2011.02.009
关键词
Carbon monoxide; CORM-3; Platelet; Rat; Soluble guanylate cyclase; Nitric oxide; Antithrombotic
资金
- Zydus Research Centre
Carbon monoxide (CO) like nitric oxide (NO) has been recognized as activator of soluble guanylate cyclase (sGC) in many physiological functions. Studies, which demonstrate the mechanisms by which CO inhibits platelet aggregation in in vivo models, are few. Here we investigated the possible involvement of sGC, NO, plasminogen activator inhibitor (PAI-1) and p38 MAP Kinase in antithrombotic effects of CO released by a novel, water-soluble, CO releasing molecule-3 (CORM-3) using rat. The effects of CORM-3 on in vitro and ex vivo platelet aggregation induced by thrombin as well as in in vivo thrombosis models were studied. When added to rat washed platelets in in vitro study, CORM-3 (100 and 200 mu M) inhibited thrombin-induced platelet aggregation. Similarly, antiplatelet effect was also observed when 3 mg/kg i.v. infusion of CORM-3 administered for 10 minutes in ex vivo study using rat. Interestingly, in presence of inhibitor of sGC (ODQ, 10 mg/kg, i.p.) and inhibitor of nitric oxide synthase (L-NAME, 30 mg/kg, i.p.), inhibition of thrombin-induced aggregation by CORM-3 was significantly blocked. Notably, in presence of inhibitor of K-ATP channel (glibenclamide, 10 mg/kg, i.p.) and p38 MAP Kinase (SCIO-469, 1 mg/kg, i.p.), inhibition of aggregation by CORM-3 was not blocked. In in vivo studies using animal models of thrombosis, we found that CORM-3-mediated antithrombotic effect was dependent on activation of sGC, NO and suppression of PAI-1 in arterial thrombosis and Arterio-Venous (A-V) shunt models. Therefore, we concluded that antithrombotic activity of CORM-3 may be mediated by activation of sGC, NO and inhibition of PAI-1. (C) 2011 Elsevier Ltd. All rights reserved.
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