期刊
THROMBOSIS AND HAEMOSTASIS
卷 104, 期 6, 页码 1184-1192出版社
GEORG THIEME VERLAG KG
DOI: 10.1160/TH10-05-0308
关键词
Platelets; lymphocytes; platelet-lymphocyte conjugates; arterial flow; thrombosis
资金
- Swedish Research Council
- Swedish Heart-Lung Foundation
- National Natural Science Foundation of China [30700267]
- Natural Science Foundation of Zhejiang Province [Y206095]
- Karolinska Institute
- Swedish Society of Medicine
- Stockholm County Council
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 +/- 3 cells/mm(2) of platelet-depleted blood to 38 +/- 11 cells/mm(2) of platelet-containing blood at the arterial shear rate of 500 s(-1). Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
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