期刊
THIN SOLID FILMS
卷 562, 期 -, 页码 653-658出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2014.05.002
关键词
Carbon ionic liquid electrode; Graphene; Nickel oxide; Myoglobin; Direct electrochemistry; Electrocatalysis
类别
资金
- National Natural Science Foundation of China [21365010, 51372125]
- Natural Science Foundation of Hainan Province [213015]
- Foundation of Hainan Normal University
By using ionic liquid 1-hexylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode, graphene (GR) and nickel oxide (NiO) were in situ electrodeposited step by step to get a NiO/GR nanocomposite modified CILE. Myoglobin (Mb) was further immobilized on the surface of NiO/GR/CILE with a Nafion film to get the electrochemical sensor denoted as Nafion/Mb/NiO/GR/CILE. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared in pH 3.0 phosphate buffer solution with the formal peak potential (E-0') located at -0.188 V (vs. SCE), which was the typical characteristics of Mb Fe(III)/Fe(II) redox couples. So the direct electron transfer of Mb was realized and promoted due to the presence of the NiO/GR nanocomposite on the electrode. Based on the cyclic voltammetric data, the electrochemical parameters of Mb on the modified electrode were calculated. The Mb modified electrode showed an excellent electrocatalytic activity towards the reduction of different substrates including trichloroacetic acid and H2O2. Therefore a third-generation electrochemical Mb biosensor based on NiO/GR/CILE was constructed with good stability and reproducibility. (C) 2014 Elsevier B.V. All rights reserved.
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