期刊
THIN SOLID FILMS
卷 519, 期 11, 页码 3925-3930出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2011.01.342
关键词
Au nanoparticles; Hemoglobin; Prussian blue; Direct electrochemistry; Hydrogen peroxide
类别
资金
- National Natural Science Foundation of China [20805040]
- Program for Science & Technology Innovation Talents in Universities of Henan Province [2010HASTIT025]
- Excellent Youth Foundation of Henan Scientific Committee [104100510020]
Direct electrochemistry of hemoglobin (Hb) is achieved by immobilizing Hb-chitosan on a gold colloid/3-aminopropyl triethylene silane/Prussian blue composite film-modified glassy carbon electrode. The modified electrode exhibits a pair of well defined and quasi-reversible peaks with a formal potential of 0.205 V. The immobilized Hb retains its biological activity and shows high catalytic activity to the reduction of hydrogen peroxide. Experimental conditions influencing the biosensor performances such as pH and potential are optimized and assessed. Under the optimized conditions, the catalytic currents are linear to the concentrations of H2O2 in the ranges of 2-480 mu M. The detection limit is 0.1 mu M (S/N = 3). The electrochemical sensor has high stability and good reproducibility. Ultraviolet visible absorption spectra and Fourier transform infrared spectroscopy show that Hb keeps almost natural structure in the composite film. Therefore, the composite film is an alternative matrix for protein immobilization and biosensor preparation. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据