期刊
THIN SOLID FILMS
卷 518, 期 2, 页码 634-637出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2009.07.053
关键词
Azurin; Au-dots; Electrochemical scanning tunneling microscopy; Conduction; Nanobiochip
类别
资金
- Ministry, Science and Technology (MEST) [M10536090001-05 N3609-00110]
- Korean government (MEST) [2006-05374]
- Ministry of Knowledge Economy (MKE)
- Korea Industrial Technology Foundation (KOTEF)
- National Research Foundation of Korea [2006-05374] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Highly dense and uniform protein dot arrays on Au-nanodots using size controllable method were fabricated on indium tin oxide (ITO) substrate in order to develop an electrochemical nanobiochip. Cysteine modified azurin was directly immobilized on the fabricated Au-nanodots without any linker materials. Atomic force microscopy was used for characterizing Au-dots formed on ITO substrate. Electrochemical scanning tunneling microscopy (ECSTM) revealed the monolayer formation with an in situ cyclic voltammetry to observe redox behaviour of both bare Au-dots and protein immobilized Au-dots. I-V characteristics were obtained on both bare Au-dots and protein immobilized Au-dots structured on ITO conductive electrodes. (C) 2009 Elsevier B.V. All rights reserved.
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