期刊
THERMOCHIMICA ACTA
卷 493, 期 1-2, 页码 30-36出版社
ELSEVIER
DOI: 10.1016/j.tca.2009.03.017
关键词
Calf thymus deoxyribonucleic acid; Isothermal titration calorimetry; Circular dichroism; Ultraviolet absorption spectra; Fluorescence spectroscopy
资金
- National Natural Science Foundation of China [20773059]
The interactions of calf thymus deoxyribonucleic acid (ct-DNA) with two antitumour drugs (5-fluorouracil and tegafur) in aqueous buffer solution (pH 7.40) have been investigated using nano-watt-scale isothermal titration calorimetry (ITC), circular dichroism (CD), ultraviolet absorption (UV) and fluorescence spectroscopy. Thermodynamic parameters, i.e., binding proportions and constants, standard changes of enthalpy (Delta H degrees), Gibbs free energy (Delta G degrees) and entropy (Delta S degrees) have been derived from the calorimetric data. The binding ratios of 5-fluorouracil and tegafur with base pairs in ct-DNA are 1:3 and 1:4, respectively. The thermodynamic parameters have been discussed according to the influence of drugs on molecular structure of the DNA shown spectrogram. The results indicate that molecule of 5-fluorouracil or tegafur can intercalate itself into the intra-molecular space formed by DNA double helix and cause some changes in the secondary structure of DNA molecule. (C) 2009 Elsevier B.V. All rights reserved.
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