期刊
THERIOGENOLOGY
卷 122, 期 -, 页码 94-101出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2018.09.010
关键词
Chicken; Sperm motility; Sequencing; MicroRNA
资金
- National Natural Science Foundation of China [31672406, 313672406]
- Beijing Municipal Science and Technology Project [D161100007817005]
- China Agriculture Research Systems [CARS-40]
- Agricultural Science and Technology Innovation Program [ASTIPIAS04, CAAS-XTCX2016010-03]
Sperm motility is one of the most important indicators for evaluating roosters' fecundity. However, molecular regulation underlying chickens' sperm motility remains to be understood. MicroRNA (miRNA) plays an epigenetic role in reproduction. In this study, we compared the testicular miRNAs of Beijing-you roosters with high (HS) and low (LS) sperm motility using Illumina sequencing to try to understand the associated molecular regulation. Length distribution analysis showed that the dominant size of small RNAs detected in the testes of the chicken samples was 24-28 nt. In total, 518 known and 106 novel miRNAs were identified. A total of 23 miRNAs were found differentially expressed (P < 0.05, vertical bar log(2)fold change vertical bar >= 1) between the HS and LS groups, including 18 known and 5 novel miRNAs. Functional enrichment of the predicted target genes of the differentially expressed miRNAs indicated that these miRNAs were involved in the pathways of GnRH, MAPK and Wnt signaling. The miRNA-gene interaction network revealed two key candidate miRNA-gene pairs that might affect chicken sperm motility, viz, gga-miR-155/KCNAI and gga-miR-7480-5p/AH11. qPCR was then used to further validate their expressions. The results here provided a deep insight into the expressions of the miRNAs in the testes of chickens and suggested their roles in sperm motility regulation. (C) 2018 Elsevier Inc. All rights reserved.
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