期刊
THERIOGENOLOGY
卷 72, 期 4, 页码 584-589出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2009.04.015
关键词
Cattle; Cytoplasm color; In vitro embryo production; Lipid contents; Mitochondria
资金
- ARPC [108068-03-1SB010]
- KOSEF [10525010001-05N2501-00110]
- Ministry of Education, Science and Technology, South Korea
The objective was to evaluate the effect of cytoplasmic lipid content on the embryonic developmental efficiency of bovine in vitro embryo production (IVP) embryos. Ovaries from Korean native cows (Bos taurus coreanae) were collected from a local abattoir, and cumulus-oocyte complexes (COCs) were recovered from follicles 2 to 8 mm in diameter. The oocytes were divided into three groups, dependent on their cytoplasm color: pale color (PC), brown color (BC), and dark color (DC). The COCs were fertilized using frozen-thawed semen from a single Hanwoo bull. Based on measurement of the cytoplasmic color intensity of oocytes after 22 h of in vitro maturation (IVM), the DC group had lower (P < 0.05) color intensity than that in the BC and PC groups (56.3 +/- 2.7, 93.3 +/- 5.1, and 123.9 +/- 12.0, respectively). Based on MitoTracker Green FM staining, the number of mitochondria in the DC (170.1 +/- 31.2) group was significantly higher than that in the BC (137.5 +/- 30.8) and PC (105.5 +/- 25.3) groups. The cleavage rate in the DC (81.5%) group was also higher than that in the PC (50.4%) group (P < 0.05), as was the development rate to blastocyst stage (18.9% vs. 9.8%). Finally, cell numbers of blastocysts in the DC (150.8 +/- 28.0) group were higher (P < 0.05) than that in the BC (107.6 +/- 17.8) and PC (80.5 +/- 12.3) groups. In conclusion, cytoplasm color was a useful selection parameter for abattoir-derived oocytes destined for IVR (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据