期刊
THERIOGENOLOGY
卷 71, 期 3, 页码 450-457出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2008.08.012
关键词
Osteopontin; Embryo development; IVF; Sperm capacitation; Cattle
Fertility-related phosphoprotein osteopontin (OPN) is present in the bovine oviduct epithelium and fluid. The objectives were to determine the effects of OPN on percentages of cleavage and embryo development in vitro in cattle, and to assess the ability of OPN to induce in vitro capacitation of bovine sperm. In vitro-matured bovine oocytes were fertilized in the presence of 0, 10, 20, or 40 mu g/mL OPN. There were greater percentages (P < 0.01) of cleavage and compact morulae-blastocysts (79.7 and 43.3%, respectively) with 10 mu g/mL OPN than in the control group (without OPN; 71.2 and 32.1%, respectively). Furthermore, percentages of advanced blastocysts were greater in the group receiving 40 mu g/mL OPN versus control (56.4% vs. 42.0%. P < 0.05). Capacitation was assessed by the ability of sperm to undergo the acrosome reaction after incubation with lysophosphatidylcholine. Semen from three bulls was incubated for 2 h in either TALP medium alone (control) or with TALP medium containing 0.01 mM heparin, or with TALP medium containing 10 or 20 mu g/mL OPN. Incubation with 10 and 20 mu g/mL OPN produced more (P < 0.01) capacitated sperm (14.4 and 13.6%, respectively) than the untreated control group (8.3%), but both untreated sperm and those treated with OPN had significantly fewer capacitated sperm than those treated with 0.01 mM of heparin (30.5%). In conclusion, OPN improved the efficiency of bovine in vitro embryo production and influenced sperm capacitation. (C) 2009 Elsevier Inc. All rights reserved.
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