期刊
THEORETICAL AND APPLIED GENETICS
卷 126, 期 8, 页码 2073-2080出版社
SPRINGER
DOI: 10.1007/s00122-013-2119-4
关键词
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资金
- NPZ Company
- Key Projects in the National Science Technology [2010BAD01B02]
- 111 project [B12006]
- NSFC [31171585]
- CSTC [201180001]
- Graduates Science and Technology Innovation Fund of Southwest University, China [Kb2011006]
- National Key Laboratory of Crop Genetic Improvement, China
Brassica rapa (AA) has been used to widen the genetic basis of B. napus (AACC), which is a new but important oilseed crop worldwide. In the present study, we have proposed a strategy to develop new type B. napus carrying genomic components of B. rapa by crossing B. rapa with hexaploid (AACCCC) derived from B. napus and B. oleracea (CC). The hexaploid exhibited large flowers and high frequency of normal chromosome segregation, resulting in good seed set (average of 4.48 and 12.53 seeds per pod by self and open pollination, respectively) and high pollen fertility (average of 87.05 %). It was easy to develop new type B. napus by crossing the hexaploid with 142 lines of B. rapa from three ecotype groups, with the average crossability of 9.24 seeds per pod. The genetic variation of new type B. napus was diverse from that of current B. napus, especially in the A subgenome, revealed by genome-specific simple sequence repeat markers. Our data suggest that the strategy proposed here is a large-scale and highly efficient method to introgress genomic components of B. rapa into B. napus.
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