期刊
THEORETICAL AND APPLIED GENETICS
卷 126, 期 2, 页码 307-315出版社
SPRINGER
DOI: 10.1007/s00122-012-1919-2
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资金
- AAFC Consortium for Blackleg Resistance (Advanta Canada Inc.)
- AAFC Consortium for Blackleg Resistance (Agriculture Victoria Services Pty. Ltd.)
- AAFC Consortium for Blackleg Resistance (Canola Breeders of Western Australia)
- AAFC Consortium for Blackleg Resistance (DNA LandMarks Inc.)
- AAFC Consortium for Blackleg Resistance (Dow AgroSciences Canada Inc.)
- AAFC Consortium for Blackleg Resistance (Monsanto Canada Seeds Inc.)
- AAFC Consortium for Blackleg Resistance (Pioneer Hi-Bred, RAPOOL-RING Ltd.)
- AAFC Consortium for Blackleg Resistance (Svalof Weibull Ltd.)
- AAFC Consortium for Blackleg Resistance (University of Manitoba)
- AAFC Matching Investment Initiative
Blackleg, caused by Leptosphaeria maculans, is one of the most economically important diseases of Brassica napus worldwide. Two blackleg-resistant lines, 16S and 61446, were developed through interspecific hybridization between B. napus and B. rapa subsp. sylvestris and backcrossing to B. napus. Classical genetic analysis demonstrated that a single recessive gene in both lines conferred resistance to L. maculans and that the resistance alleles were allelic. Using BC1 progeny derived from each resistant plant, this locus was mapped to B. napus linkage group N6 and was flanked by microsatellite markers sN2189b and sORH72a in an interval of about 10 cM, in a region equivalent to about 6 Mb of B. rapa DNA sequence. This new resistance gene locus was designated as LepR4. The two lines were evaluated for resistance to a wide range of L. maculans isolates using cotyledon inoculation tests under controlled environment conditions, and for stem canker resistance in blackleg field nurseries. Results indicated that line 16S, carrying LepR4a, was highly resistant to all isolates tested on cotyledons and had a high level of stem canker resistance under field conditions. Line 61446, carrying LepR4b, was only resistant to some of the isolates tested on cotyledons and was weakly resistant to stem canker under field conditions.
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