期刊
THEORETICAL AND APPLIED GENETICS
卷 124, 期 3, 页码 585-596出版社
SPRINGER
DOI: 10.1007/s00122-011-1731-4
关键词
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资金
- National '863' High-Tech Program of China
- National '973' Basic Research Program [2009CB118401]
Stalk rot is one of the most devastating diseases in maize worldwide. In our previous study, two QTLs, a major qRfg1 and a minor qRfg2, were identified in the resistant inbred line '1145' to confer resistance to Gibberella stalk rot. In the present study, we report on fine-mapping of the minor qRfg2 that is located on chromosome 1 and account for similar to 8.9% of the total phenotypic variation. A total of 22 markers were developed in the qRfg2 region to resolve recombinants. The progeny-test mapping strategy was developed to accurately determine the phenotypes of all recombinants for fine-mapping of the qRfg2 locus. This fine-mapping process was performed from BC4F1 to BC8F1 generations to narrow down the qRfg2 locus into similar to 300 kb, flanked by the markers SSRZ319 and CAPSZ459. A predicted gene in the mapped region, coding for an auxin-regulated protein, is believed to be a candidate for qRfg2. The qRfg2 locus could steadily increase the resistance percentage by similar to 12% across different backcross generations, suggesting its usefulness in enhancing maize resistance against Gibberella stalk rot.
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