期刊
THEORETICAL AND APPLIED GENETICS
卷 121, 期 5, 页码 971-984出版社
SPRINGER
DOI: 10.1007/s00122-010-1366-x
关键词
-
资金
- Indian Council of Agriculture (ICAR)
- Department of Biotechnology (DBT) of the Government of India
- Tropical Legume I of Generation Challenge Programme
Late leaf spot (LLS) and rust are two major foliar diseases of groundnut (Arachis hypogaea L.) that often occur together leading to 50-70% yield loss in the crop. A total of 268 recombinant inbred lines of a mapping population TAG 24 x GPBD 4 segregating for LLS and rust were used to undertake quantitative trait locus (QTL) analysis. Phenotyping of the population was carried out under artificial disease epiphytotics. Positive correlations between different stages, high to very high heritability and independent nature of inheritance between both the diseases were observed. Parental genotypes were screened with 1,089 simple sequence repeat (SSR) markers, of which 67 (6.15%) were found polymorphic. Segregation data obtained for these markers facilitated development of partial linkage map (14 linkage groups) with 56 SSR loci. Composite interval mapping (CIM) undertaken on genotyping and phenotyping data yielded 11 QTLs for LLS (explaining 1.70-6.50% phenotypic variation) in three environments and 12 QTLs for rust (explaining 1.70-55.20% phenotypic variation). Interestingly a major QTL associated with rust (QTL(rust)01), contributing 6.90-55.20% variation, was identified by both CIM and single marker analysis (SMA). A candidate SSR marker (IPAHM 103) linked with this QTL was validated using a wide range of resistant/susceptible breeding lines as well as progeny lines of another mapping population (TG 26 x GPBD 4). Therefore, this marker should be useful for introgressing the major QTL for rust in desired lines/varieties of groundnut through marker-assisted backcrossing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据