期刊
THEORETICAL AND APPLIED GENETICS
卷 118, 期 2, 页码 359-370出版社
SPRINGER
DOI: 10.1007/s00122-008-0905-1
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Understanding plant host response to a pathogen such as Phakopsora pachyrhizi, the causal agent of Asian soybean rust (ASR), under different environmental conditions and growth stages is crucial for developing a resistant plant variety. The main objective of this study was to perform global transcriptome profiling of P. pachyrhizi-exposed soybean ( Glycine max) with susceptible reaction to the pathogen from two distinct developmental growth stages using whole genome Affymetrix microarrays of soybean followed by confirmation using a resistant genotype. Soybean cv. 5601T ( susceptible to ASR) at the V-4 and R-1 growth stages and Glycine tomentella ( resistant to ASR) plants were inoculated with P. pachyrhizi and leaf samples were collected after 72 h of inoculation for microarray analysis. Upon analyzing the data using Array Assist software at 5% false discovery rate (FDR), a total of 5,056 genes were found significantly differentially expressed at V-4 growth stage, of which 2,401 were up-regulated, whereas 579 were found differentially expressed at R-1 growth stage, of which 264 were up-regulated. There were 333 differentially expressed common genes between the V-4 and R1 growth stages, of which 125 were up-regulated. A large difference in number of differentially expressed genes between the two growth stages indicates that the gene expression is growth-stage-specific. We performed real-time RT-PCR analysis on nine of these genes from both growth stages and both plant species and found results to be congruent with those from the microarray analysis.
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