期刊
TETRAHEDRON LETTERS
卷 55, 期 4, 页码 873-876出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2013.12.033
关键词
Cu2+-selectivity; Fluorescent probe; Coumarin derivative; Molecular dynamics; Lipid bilayer
资金
- FONDEF [VIU-110063]
- FONDECYT [1130141]
In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties. (C) 2013 Elsevier Ltd. All rights reserved.
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