期刊
TETRAHEDRON LETTERS
卷 54, 期 23, 页码 2999-3002出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2013.03.120
关键词
Cytochalasin; Biosynthesis; Polyketide synthase; Non-ribosomal peptide synthetase; Fungal metabolite
资金
- Japan Society for the Promotion of Science (JSPS) [22108002]
- JSPS
- Grants-in-Aid for Scientific Research [22108007] Funding Source: KAKEN
Cytochalasins are an important class of fungal natural products in view of structural diversity and biological activities. Although their biosynthetic studies have been examined extensively, the detailed molecular assembly mechanism remains to be solved. We have succeeded to heterologously express the cytochalasin polyketide synthase-non-ribosomal peptide synthetase (PKS-NRPS) hybrid gene ccsA and the trans-acting enoyl-CoA reductase gene ccsC in Aspergillus oryzae. The resultant transformant produced a novel metabolite possessing the cytochalasin backbone. This established that CcsA is capable of constructing the octaketide connected with phenylalanine in collaboration with CcsC, and that CcsA R domain catalyzes reductive cleavage of the thio-tethered PKS-NRPS product. (C) 2013 Elsevier Ltd. All rights reserved.
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