期刊
TETRAHEDRON LETTERS
卷 52, 期 23, 页码 3048-3053出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2011.04.026
关键词
Fluorescent probes; ESIPT; Succinimidyl dyes; Epifluorescence; Candida albicans
资金
- CNPq
- FAPERGS
New fluorescent succinimidyl benzazole derivatives were synthesised and successfully used to stain Candida albicans ATCC 10231 cells. The dyes were characterised by means of infrared, C-13 and H-1 NMR spectroscopies and elemental analysis. UV-Vis and steady-state fluorescence in solution were also applied to characterise their photophysical behaviour. The novel dyes were fluorescent in the yellow-green region by a phototautomerism in the excited state (ESIPT) with a large Stokes shift (9065-10962 cm(-1)). Dual fluorescence could also be observed depending on the solvent polarity. The present dyes were used as new probes by means of culture methodology or direct staining to study the micromorphology of Candida albicans. (C) 2011 Elsevier Ltd. All rights reserved.
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