期刊
TETRAHEDRON LETTERS
卷 52, 期 49, 页码 6606-6609出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2011.09.137
关键词
Biothiols; Coumarin-based derivatives; Fluorescence probes; Michael addition
资金
- FONDECYT [11090115, 1100640]
- Millennium Scientific Initiative [P05-001-F]
Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric thiol determination in SH-SY5Y cells. (C) 2011 Elsevier Ltd. All rights reserved.
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