期刊
TETRAHEDRON
卷 67, 期 23, 页码 4206-4211出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2011.04.055
关键词
Biotransformation; Pentacyclic triterpene; Glycosylation; Oxidation; Streptomyces griseus ATCC 13273; Aspergillus ochraceus CICC 40330
资金
- National Nature Science Foundation of China (NSFC) [20602040, 30672603]
- State Administration of Foreign Expert Affairs of China [111-2-07]
- Ministry of Education of China
Microbial transformation of 4 olean-type pentacyclic triterpenes (OPTs), 3-oxo oleanolic acid (1), 3-acetyl oleanolic acid (2), oleanolic acid (3), and esculentoside A (4) was studied. After the screening of 12 strains of microbes, preparative biotransformation by two strains of Streptomyces griseus ATCC 13273 and Aspergillus ochraceus CICC 40330 resulted in the isolation of 10 metabolites. The microbial catalyzed high efficient regio-selective methyl oxidation and glycosylation were discovered, which could be provided as an alternative method to expand the structural diversity of OPTs. All the structures of the metabolites were elucidated unambiguously by ESI-MS, H-1 NMR, C-13 NMR, and 2D-NMR spectroscopy. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据