期刊
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
卷 9, 期 1, 页码 77-86出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/153303461000900109
关键词
Taxol; MicroRNA-21; PAMAM dendrimer; Breast carcinoma
类别
资金
- China National Natural Scientific Fund [50573056, 30971136, 30772231, 30670802]
- ministry of science and technology of China [2009CB918903]
- Tianjin Science and Technology Committee [09JCZDJC17600, 09JCZDJC19700]
- New Century Excellent Talents in University [NCET-07-0615]
The successful of anti-cancer treatment are often limited by the development of drug resistance. Recent work has highlighted the involvement of non-coding RNAs, microRNAs(miRNAs) in cancer development, and their possible involvement in the evolution of drug resistance has been proposed. In this study, we combine taxol chemotherapy and miR-21 inhibitor treatment via polyamidoamine (PAMAM) dendrimers vector to evaluate the effects of combination therapy on suppression of breast cancer cells. The 50% inhibitory concentration (IC50) values for taxol were significantly decreased to a greater extent in the cells transfected with miR-21 inhibitor compared with cells treated with taxol alone. Taxol treatment also increased the percentage of apoptotic breast cancer cells in miR-21 inhibitor transfected cells compared with control cells. Furthermore, treatment of the miR-21 inhibitor-transfected cells with the anti-cancer drugs taxol resulted in significantly reduced cell viability and invasiveness compared with control cells. These results indicated that the miR-21 plays an important role in the resistance of breast carcinoma cells to chemotherapeutic drugs. Therefore, miR-21 inhibitor gene therapy combined with taxol chemotherapy might represent a promising novel therapeutic approach for the treatment of breast malignancies.
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