期刊
TALANTA
卷 193, 期 -, 页码 93-99出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.09.093
关键词
Tyrosinase; Screen-printed electrodes; Antioxidants; Tyrosol; Beer
资金
- Junta de Extremadura, Spain (European Union Funds for Regional Development (FEDER)) [PRI IB16114, GR18068]
In this work it is explored a real applicability of miniaturised and portable biosensing technology for the estimation of total phenolic content in 15 different commercial beers by applying direct amperometry. Gold nanoparticles screen-printed electrodes were thoroughly modified with tyrosinase (Tyr-AuNPS-SPCEs), which was immobilised on the surface by crosslinking with glutaraldehyde. All chemical and instrumental variables involved in the electrochemical method were optirnised to develop a reliable and powerful tool to estimate rapidly the content of phenolic compounds in complex beer samples. Catechol, phenol, caffeic acid and tyrosol were analysed individually using the proposed methodology and good analytical and kinetic performances were obtained. Total phenolic content in tested beers (high fermented, low fermented and non-alcoholic) were expressed as mg L-1 of tyrosol, which is one of the major phenolic compound reported in beer. Moreover, the developed amperometric methodology was successfully benchmarked against standardised Folin-Ciocalteau spectrophotometric method with a good Pearson correlation (r = 0.821, p < 0.01). Hierarchical Cluster Analysis (HCA) was also applied on electrochemical results and a good capability to group tested beers based on their tyrosol concentration was demonstrated.
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