Facile fabrication of a novel anisotropic gold nanoparticle-chitosan-ionic liquid/graphene modified electrode for the determination of theophylline and caffeine

In the present study, a suspension solution containing anisotropic gold nanoparticle (GNP), chitosan (CHIT) and ionic liquid (IL, i.e. 1-butyl-3-methylimidazolium tetrafluoroborate, [BMIM][BF4]), is prepared by reducing HAuCl4 with sodium citrate in CHIT-IL aqueous solution. The hybrid solution is coated on a graphene (r-GO) modified glassy carbon electrode to construct an electrochemical sensor for the determination of theophylline (TP) and caffeine (CAF). The obtained hybrid film shows rough surface, and anisotropic GNPs are well dispersed on it. The factors concerning this assay strategy are carefully investigated, including the components of the hybrid film, the concentrations of r-GO, HAuCl4 and IL, and the pH of buffer solution. Under the optimized conditions, the linear response ranges are 2.50 x 10(-8)-2.10 x 10(-6) mol L-1 and 2.50 x 10(-8)-2.49 x 10(-6) mol L-1 for TP and CAF, respectively; the detection limits are 1.32 x 10(-9) mol L-1 and 4.42 x 10(-9) mol L-1, respectively. The electrochemical sensor shows good reproducibility, stability and selectivity, and it has been successfully applied to the determination of TP and CAF in real samples. (C) 2014 Elsevier B.V. All rights reserved.