期刊
TALANTA
卷 129, 期 -, 页码 431-437出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2014.06.007
关键词
Clenbuterol; Immunochromatographic strip; Magnetic solid phase extraction; Magnetic sulfonated polystyrene nanobeads
资金
- Twelfth Five-Year Plan for National Science and Technology Support Program [2012BAK171302, 2013BAD19802]
- National Natural Science Foundation of China [31360385]
- Jiangxi Province Department of Education [KJLD13011]
- Innovation Fund Designated for Graduate Students of Jiangxi Province [YC2013-8022]
A magnetic solid-phase extraction method (MSPE) was developed to pre-concentrate and cleanup clenbuterol (CLE) from pork muscle. Novel sulfonated polystyrene magnetic nanobeads (spMNBs) were synthesized via a one-pot emulsion copolymerization method by using divinylbenzene, styrene, and sodium styrene sulfonate in the presence of oleic acid-modified and 10-undecylenic acid-modified magnetic ferrofluid. The resulting spMNBs exhibited high adsorption efficiency for CLE and for 10 other common beta-adrenergic agonists, namely, brombuterol, ractopamine, tulobuterol, bambuterol, cimbuterol, mabuterol, clorprenaline, penbutolol, salbutamol, and cimaterol. The adsorption behavior of the spMNBs for CLE was described by the Langmuir equation with a maximum adsorption capacity of 0.41 mg/g. Under the optimized parameters, the extraction of CLE from 0.5 g of pork muscle required 25 mg of the spMNBs at a shortened adsorption time (0.5 mm). The proposed MSPE was coupled with colloidal gold nanoparticle-based immunochromatographic assay (MSPE-AuNPIA) for the quantitative detection of CLE residue in pork muscle. The limit of detection and limit of quantification for the pork muscle were 0.10 and 0.24 ng/g, respectively. The intra-day and inter-day assay recoveries at three CLE spiked concentrations ranged from 92.5% to 98.1%, with relative standard deviations ranging from 3.2% to 13.0%. The results of MSPE-AuNPIA were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The CLE values obtained with MSPE-AuNPIA agreed with those obtained with LC-MS/MS. (C) 2014 Elsevier B.V. All rights reserved.
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