Indirect competitive enzyme-linked immunosorbent assay of tris-(2,3-dibromopropyl) isocyanurate with monoclonal antibody

Tris-(2,3-dibromopropyl) isocyanurate (TBC) is a heterocyclic brominated flame retardant and posses typical characteristic of Persistent Organic Pollutants (POPs). To meet the need for rapid and reliable monitoring of TBC, a monoclonal antibody was produced and an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on the monoclonal antibody. Monoclonal antibody against TBC was generated using synthesized haptens in mice. After optimization of the immunoassay conditions, results showed that the IC50 and the limit of detection (LOD) were 1.59 and 0.06 mu g/L, respectively. The monoclonal antibody shows high specificity and the developed IC-ELISA is with high recoveries. The precision investigation indicated that the intra-assay precision values were all below 9.2% and that the inter-assay precision values ranged from 6.7 to 11.3%. The assay of real samples gives results basically consistent with UHPLC-MS/MS. The obtained results showed that this proposed immunoassay is a potential method for rapid and reliable monitoring of TBC. (C) 2014 Elsevier B.V. All rights reserved.