期刊
TALANTA
卷 120, 期 -, 页码 40-46出版社
ELSEVIER
DOI: 10.1016/j.talanta.2013.11.080
关键词
Tetrabromobisphenol A; Enzyme-linked immunosorbent assay; Biotin-streptavidin-amplified system; Electronic waste
资金
- National Natural Science Foundation of China [21177082]
- Science and Technology Commission of Shanghai Municipality in China [09JC1407600]
- science and technology innovation special fund development projects of Shanghai jiaotong university
Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant. A sensitive and selective indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for detecting TBBPA. The optimal hapten of TBBPA was 2-(2,6-dibromo-4-(2-(3,5-dibromo-4-hydroxyphenly)propan-2-yl)) acetic acid. Several physiochemical factors that influence assay performance, such as optimal coupling concentration of immunogen and antibody, organic solvent, ionic strength, and pH, were studied and optimized. The limit of detection (IC10) was 0.027 ng/mL and the median inhibitory concentration (IC50) was 0.58 ng/mL. The BA-ELISA was highly selective, with low cross-reactivity with TBBPA analogs. Finally, the assay was used to detect TBBPA in electronic waste samples. The results are consistent with those using liquid chromatography, which proves that the proposed immunoassay is accurate and receptive. This BA-ELISA method is suitable for the rapid and sensitive screening of TBBPA in environmental monitoring. (C) 2013 Elsevier B.V. All rights reserved.
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