期刊
TALANTA
卷 105, 期 -, 页码 131-134出版社
ELSEVIER
DOI: 10.1016/j.talanta.2012.11.078
关键词
Cocaine; Cytochrome P450 2B4; Screen-printed electrodes; Enzymatic biosensor
资金
- Spanish Ministry [TEC2009-12029, CENIT-E 2009-INFINITEX, INNPACTO SERIBIO 2011]
- Junta de Castilla y Leon [BU212A12-2]
- Spanish Ministry of Science, Culture and Sport
This paper describes the development of screen-printed cytochrome P450 2B4 based biosensors, from the fabrication of the device using a single screen-printing technology to its characterization and application in the determination of cocaine street samples. Voltammetric measurements were first performed in order to study the electrochemical behavior of the enzyme trapped in the structure of the working electrode, as well as its interaction with one of its substrates, cocaine. Then, chronoamperometric measurements were carried out for the characterization of the biosensors. Calibration curves under optimum conditions of the experimental variables have been used for establishing the reproducibility (5.91%, n=5), the repeatability (10.45%, n=3) and the capability of detection (0.2 mM, alpha=beta=0.05) of the described method in the calibration range from 0.2 to 1.2 mM. Finally, the performance of the developed biosensors has been successfully probed by the analysis of the purity of cocaine street samples. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据