期刊
TALANTA
卷 113, 期 -, 页码 1-6出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.03.074
关键词
Graphene; Au nanorods; NADH Ethanol biosensor
资金
- National Natural Science Foundation of China [20975115, 21105126, 21175157]
- China Hunan Provincial Science and Technology Department [2012FJ4139]
- Central South University [2010QZZD007]
- China Postdoctoral Science Foundation [2011M500126, 2012T50656, 201104511]
In this paper, a simple strategy for the synthesis of graphene-Au nanorods hybrid nanosheets (GN-AuNRs) through electrostatic interaction has been demonstrated. Due to the synergistic effect between AuNRs and GN, the hybrid nanosheets exhibited excellent performance toward dihydronicotinamide adenine dinucleotide (NADH) oxidation, with a low detection limit of 6 mu M. The linear GN-AuNRs also served as a biocompatible and electroactive matrix for enzyme assembly to facilitate the electron transfer between the enzyme and the electrode. Using alcohol dehydrogenase (ADH) as a model system, a simple and effective sensing platform was developed for ethanol assay. The response displayed a good linear range from 5 to 377 mu M with detection limit 1.5 mu M. Furthermore, the interference effects of redox active substances, such as uric acid, ascorbic acid and glucose for the proposed biosensor were negligible. (C) 2013 Elsevier B.V. All rights reserved.
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