期刊
TALANTA
卷 106, 期 -, 页码 249-254出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.10.074
关键词
Glutathione-S-transferase; Enzymatic biosensor; Voltammetry; Molinate; Rice paddy field floodwater
资金
- CAPES [313/11]
- FCT [313/11]
- POPH-QREN-Tipologia 4.1-Formacao Avancada [SFRH/BPD/78845/2011]
- Fundo Social Europeu
- Ministerio da Ciencia, Tecnologia e Ensino Superior
- Fundacao para a Ciencia e a Tecnologia (FCT) [PEst-C/EQB/LA0006/2011]
A glutathione-S-transferase (GST) based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water. The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane-glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mg L-1 and presenting a detection limit of 0.064 mg L-1. The developed biosensor is stable, and reusable during 15 days. The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据