期刊
TALANTA
卷 115, 期 -, 页码 129-132出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.04.046
关键词
Hydrophilic interaction liquid; chromatography; Amide stationary phase; Ion chromatography; Click chemistry
资金
- National Science and Technology Major Science Instrument Project of the Ministry of Science and Technology of China [2012YQ090229]
- National Natural Science Foundation of China [21075038, 21177040]
A novel amide stationary phase (ASP) for hydrophilic interaction liquid chromatography (HILIC) has been prepared via the Click chemistry method. It was based on the strategy that the amino group of Asparagine was easily transferred to the corresponding azido group and then clicked onto terminal alkyne-silica gel in the presence of Cu(I)-based catalyst. For the tested polar compounds including nucleosides and nucleic acid bases, ASP-based column has demonstrated good performance in terms of separation efficiency and column stability, and the retention mechanism was found to match well the typical HILIC retention. In addition, the ASP described here showed much better selectivity in separation of inorganic anions under ion chromatography mode relative to other kinds of commercial ASP. (c) 2013 Elsevier B.V. All rights reserved.
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