期刊
TALANTA
卷 117, 期 -, 页码 196-202出版社
ELSEVIER
DOI: 10.1016/j.talanta.2013.09.003
关键词
Hydrothermal refluxing; Tris; Lactose; Fluorescence; Carbon dots; Imaging
资金
- National Basic Research Program of China [2011CB707703, 2012CB910601]
- National Natural Science Foundation of China [21075069, 21275078]
- Tianjin Natural Science Foundation [11JCZDJC21700]
Due to their unique optical and biochemical properties, the water-soluble fluorescent carbon dots (CDs) have attracted a lot of attention recently. Here, strong fluorescent carbon dots with excellent quality have been synthesized by the hydrothermal refluxing method using lactose as carbon source and tris (hydroxymethyl) aminomethane (i.e. Tris) as surface passivation reagent. This facile approach was simple, efficient, economical, green without pollution, and allows large-scale production of CDs without any post-treatment. TEM measurements showed that the resulting particles exhibited an average diameter of 1.5 nm. The obtained CDs possess small particle sizes, good stability in a wide range of pH values (pH 3.5-9.5), high tolerance of salt concentration, strong resistibility to photobleaching, and a fluorescent quantum yield up to 12.5%. The CDs were applied to optical bioimaging of HeLa cells, showing low cytotoxicity and excellent biocompatibility. (C) 2013 Elsevier B.V. All rights reserved.
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