期刊
TALANTA
卷 116, 期 -, 页码 951-957出版社
ELSEVIER
DOI: 10.1016/j.talanta.2013.08.010
关键词
Fluorescent sensor; Gold nanoparticle; Fluorescein; Protamine; Heparin
资金
- National Natural Science Foundation of China [21275051, 21075037]
- Scientific Research Fund of Hunan Provincial Education Department [12A084]
- Science and Technology Department [13JJ2020]
- Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
Here we report a novel label-free fluorescent sensor for ultrasensitive detection of protamine and heparin based on the high quenching ability of gold nanoparticles to the fluorescence of fluorescein. The fluorescence was significantly quenched when fluorescein molecules were attached to the surface of gold nanoparticles by electrostatic interaction. Upon addition of protamine, the fluorescein molecules were detached from the surface of the gold nanoparticles due to the stronger adsorption of protamine on the surface of AuNPs, and resulting in the recovery of the fluorescein molecules fluorescence. Heparin is able to bind with Protamine specifically. In the presence of heparin, the interaction of heparin with protamine makes the AuNPs de-aggregate and the fluorescein molecules re-attach to the AuNPs, which lead to marked fluorescence quench again. By measuring the changes in the fluorescence of the fluorescein molecules, the concentration of protamine and heparin were sequentially determined. The linear response range was obtained over the concentration range from 0 to 0.8 mu g/mL and 4 to 1.6 mu g/mL with the low detection limit 0.0067 mu g/mL and 0.0013 mu g/mL for protamine and heparin, respectively. (C) 2013 Published by Elsevier B.V.
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