期刊
TALANTA
卷 109, 期 -, 页码 160-166出版社
ELSEVIER
DOI: 10.1016/j.talanta.2013.02.003
关键词
Carbon nanotube; Multiplexed detection; Nanobeacon; DNA
资金
- National Natural Science Foundations of China [21175030]
- Natural Science Foundations of Guangxi Province [2010GXNSFF013001]
A highly sensitive and selective multi-walled carbon nanotube (MWCNT)-based multicolor fluorescent nanobeacon is developed for multiplexed analysis of DNA in homogeneous solution. In this work, three different dye-labeled DNA hairpins were adsorbed on MWCNTs surface via pi-stacking, which brings the dyes and MWCNTs into close proximity and leads to the quenching of fluorescence of the dyes. When target DNAs were added to the solution, the target DNAs specifically hybridize with the probes to form stable DNA duplexes, which weakens the interactions between the probes and MWCNTs, and results in the fluorescence recovery of the dyes. By using three 15-mer DNA fragments as proof-of-principle analytes, the proposed method showed good analytical performance. The limits of detection obtained were in the range of 35-42 pM. Moreover, this method also exhibits an excellent ability to discriminate between single nucleotide polymorphisms. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据