期刊
TALANTA
卷 104, 期 -, 页码 97-102出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.11.041
关键词
Clenbuterol; Salbutamol; Terbutaline; Formoterol; CE-ESI-MS; FASS
资金
- National Nature Sciences Foundation of China [21075016, 21275029, 21005018]
- Program of the Industrial Technology Development of Fujian Province
- Program for Changjiang Scholars and Innovative Research Team in University, China [IRT1116]
Field amplified sample stacking (FASS) was combined with a simple, rapid, sensitive CE-ESI-MS method to achieve the on-line enrichment and simultaneous determination of Clenbuterol (CLE), Salbutamol (SAL), Terbutaline (TER) and Formoterol (FOR). Samples were diluted in deionized water, and electrokinetic injection (10 kV x 50 s) was employed to carry out FASS. With FASS, the four beta(2)-agonists had simultaneously baseline enhancement as much as 319, 332, 297 and 115 fold, respectively. Consequently, satisfactory LODs (S/N=3) of 0.08, 0.1, 0.1 and 0.5 ng/mL for CLE, SAL, TER and FOR were obtained. The separation of the four analytes was performed at 22 kV in ammonium acetate/ammonia (20 mmol/L, pH 9.0), using 7.5 mmol/L acetic acid in isopropanol/water 50/50% (v/v) as sheath liquid. In addition, an excellent linear response was obtained with RSD less than 1.3% for migration times and less than 6.7% for peak areas (n=5). The recoveries of spiked urine samples were in the range of 82.7-101% with RSD lower than 9.8%. The proposed method has been applied to analyze human urine samples successfully. (C) 2012 Elsevier B.V. All rights reserved.
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