期刊
TALANTA
卷 93, 期 -, 页码 172-181出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.02.008
关键词
Molecular imprinted polymers; Rutin; Solid-phase extraction; Bi-functional monomer; Bi-crosslinker; Chinese medicinal plants
资金
- National Natural Science Foundation of China [21175040]
Molecular imprinted polymers (MIPs) were prepared using rutin as the template, different reagents as the functional monomer and different reagents as the cross-linker by solution polymerization. Several parameters that would influence the performance of MIPs were investigated including the type of functional monomer (single or double) and cross-linker (single or double), and the molar ratio of the template, the functional monomer and the cross-linker. The optimum synthesis conditions of MIPs were found to be bi-monomers (acrylamide-co-2-vinyl pyridine, 3:1) and bi-crosslinker (ethylene glycol dimethacrylate-co-divinylbenzene, 3:1). The ratio of the template, the functional monomer and the cross-linker was found to be 1:6:20. MIPs synthesized under these conditions were filled into the cartridges as the adsorbents of solid-phase extraction (SPE). A competition test was conducted to authenticate the selectivity and the specificity of molecularly imprinted solid-phase extraction (MISPE) for rutin using the mixture solution of standard rutin and its structural analogs including quercetin, naringenin and kaempferol. Compared with purchased SPE including C-18, silica and PCX, MISPE showed better selectivity and enrichment property for rutin in the extracted solutions of Chinese medicinal plants than any others. The mean recoveries were 85.93% (RSD: 3.04%, n = 3) for Saururus chinensis (Lour.) Bail and 88.61% (RSD: 3.36%, n = 3) for Flos Sophorae, respectively, which indicated that the optimized rutin-MIPs possess the value of practical application. (C) 2012 Elsevier B.V. All rights reserved.
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